Regulation of MMP-9 by p53 in first trimester cytotrophoblastic cells -- Cohen et al., 10.1093 humrep den264 -- Human Reproduction
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For commercial re-use, please contact journals.permissions oxfordjournals.org Law of MMP-9 by p53 in beginning trimester cytotrophoblastic cells M. Cohen 1, C. Wuillemin, O. Irion and P.
Bischof Branch of Obstetrics and Gynaecology, Maternity, Laboratory of Hormonology, University of Geneva, 30 Boulevard de la Cluse, 1211, Geneva 14, Switzerland 1 Mail address.
Tel: +41-22-38-24-381; Fax: +41-22-38-24-310; E-mail: marie.cohen at hcuge.ch BACKGROUND: The matrix metalloproteinase (MMP) family is acknowledged to play a explanation role in tissue remodelling during embryonic transaction and in pathological conditions, such as cardiovascular disease, arthritis and cancer metastasis.
It has been shown formerly that p53 regulates positively or negatively the expression of at odds MMPs.
In that of p53 overexpression in trophoblastic cells, and its conceivable role in regulating MMP-2 and MMP-9 locution in contrasting cell lines, we hypothesized that the word of MMP-9 could too be regulated by p53 in antecedent trimester cytotrophoblasts (CTB).
METHODS and RESULTS: Transfection experiments in CTB demonstrated that wild-type p53 down-regulates the -670 ( P « 0.001) however not the -531 and -90 human MMP-9 promoter CAT journalist plasmid activity, whereas p53 mutants partially irretrievable this repressive activity. However, endogenous p53 is not able to open MMP-9 term in CTB. The presence of gigantic molecular weight complexes of p53 in CTB suggests a budding mechanism of inactivation of p53 transcriptional hustle in relation to MMPs in these cells.
CONCLUSIONS: Although p53 is mutated in trophoblast, it is functionally incompetent towards MMPs in these cells. Gloss words: matrix metalloproteinase-2 matrix metalloproteinase-9 p53 front trimester cytotrophoblast gelatinase Submitted on Feb 26, 2008; resubmitted on May 9, 2008; universal on Jun 9, 2008. CiteULike Connotea Del.icio.us What's this?
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