Maternal diabetes adversely affects AMP-activated protein kinase activity and cellular metabolism in murine oocytes -- Ratchford et al. 293 (5): E1198 -- AJP - Endocrinology and Metabolism
M. Articles by Moley, K. H. PubMed PubMed Quotation Articles by Ratchford, A. H. Maternal diabetes adversely affects AMP-activated protein kinase action and cellular metabolism in murine oocytes Ann M. Ratchford, Aimee S. Chang, Maggie M.- Y. Chi, Rachael Sheridan, and Kelle H. Moley Branch of Obstetrics and Gynecology, Washington University in St.
Louis, St. Preovulatory oocytes in murine models further acquaintance maturational delay and worthier granulosa cell apoptosis. The straight of this peruse was to scrutinize if maternal diabetes influences preovulatory oocyte metabolism and impacts meiotic maturation.
ATP levels were significantly lower in oocytes from diabetic mice, and the percent replace in the AMP-to-ATP ratio was significantly higher in these oocytes. In contrast, activities of Hadh2 and Gpt2, two enzymes activated by AMPK, were significantly less in these oocytes. Additionally, glycogen and FBP levels, both endogenous inhibitors of AMPK, were elevated.
Phosphorylated ACC, a downstream intention of AMPK, and phosphorylated AMPK were both decreased in diabetic oocytes, thus confirming decreased AMPK activity. Finally, appendix of the activator AICAR to the in vitro maturation assay restored AMPK existence and corrected the maturation defect experienced by the oocytes from diabetic mice.
In conclusion, maternal diabetes adversely alters cellular metabolism influential to abnormal AMPK exercise in murine oocytes. Increasing AMPK exertion in these oocytes during the preovulatory event reverses the metabolic changes and corrects delays in meiotic maturation.
